polyclonal goat anti integrin α8  (R&D Systems)

Journal: The Journal of Veterinary Medical Science

Article Title: Tensin2-deficient mice on FVB/N background develop severe glomerular disease

doi: 10.1292/jvms.15-0442

Figure Lengend Snippet: Dual immunofluorescence study for a mesangial marker, integrin α8 and laminin α2 in 3-week-old female FVB and FVBGN mice. Mesangial process invasion of the glomerular capillary tufts was observed in FVBGN mice. Laminin α2 localized to the mesangium and the GBM in FVBGN mice. Arrows denote area of dual immunostaining in the glomerular capillary loop. Scale bars=50 µ m.

Article Snippet: The primary antibodies used were as follows: anti-laminin α1 rat monoclonal and anti-fibronectin rabbit polyclonal antibodies at 1:100 (Millipore, Billerica, MA, U.S.A.), anti-laminin α2, β1 and γ1 rat monoclonal and anti-CD151 rabbit polyclonal antibodies at 1:200 (Santa Cruz), anti-laminin α5 and β2 rabbit polyclonal antibodies (Sigma, St. Louis, MO, U.S.A.), anti-α1, α2, α3, α4 and α5 chains of collagen IV rat monoclonal antibodies at 1:100 (Chondrex, Redmond, WA, U.S.A.), anti-phospho-FAK rabbit polyclonal antibody at 1:100 (Abcam, Cambridge, U.K.) and anti-integrin α8 goat polyclonal antibody at 1:200 (R&D Systems, Minneapolis, MN, U.S.A.).

Techniques: Immunofluorescence, Marker, Immunostaining